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wet-Lab-Techniques

Proteome Research requires ample state-of-the-art experimental techniques for protein identification and validation as well as for detailed protein structure and structure-/function characterization.

Sample Preparation:

  • Generation of protein extracts from tissue
  • Affinity chromatography using magnetic beads
  • Anion exchange-based Stage Tip peptide fractionation
  • Phosphopeptide enrichment
  • Fractionated precipitation
  • Cell culture
  • Ultra Centrifuge (Beckman)
  • Laser Microdissection & Pressure Catapulting System (P.A.L.M. )
  • Fluorescence Microscope (Zeiss)
  • RNA Interference
  • Cytometry System FACSCalibur™ (Becton Dickinson)

Mass Spectrometry/Protein Analysis:

  • Synapt G2-S (Waters)
  • MALDI-ToF-MS (Bruker)
  • MALDI-QIT-ToF-MS (Shimadzu)
  • LC-ESI-Q-ToF-MS (Micromass)
  • Online Nano-LC (Dionex)
  • Online Nano-LC, inert system (Dionex)
  • Surface Plasmon Resonance Spectrometer (Biacore)
  • MAP II-Robot (Bruker)
  • Chemical Printer (Shimadzu)

2D-Gel Electrophoresis/Separation Methods:

  • 1D- and 2D- Electrophoresis units (Invitrogen, BioRad, GE Healthcare)
  • UMAX scanner
  • Fluorescence scanner (Fuji)
  • HPLC (Agilent)
  • Picking-Robot (Genomics solutions)
  • Automatic Digester (Genomics solutions)
  • BioCAD Sprint Perfusion Chromatography™ System (Applied Biosystems)
  • Free-Flow Electrophoresis

Transcriptomics:

  • High Density Microarray System (Affymetrix)
  • Sensi Chip Microarray Reader (Qiagen)
  • Pyrosequencer (PSQ 96) (Pyrosequencing Ltd.)
  • TaqMan (Applied Biosystems)
  • WAVE® DHPLC System (Transgenomic)
  • ABI PRISM® 310 Single-Capillary Genetic Analyzer (Applied Biosystems)
  • BioRobot8000 (Qiagen)